Verification of a Putative Caspase Substrate

MCSTAY, Gavin and Green, D. R. (2014) Verification of a Putative Caspase Substrate. Cold Spring Harbor Protocols, 2014 (8). pdb.prot080317-pdb.prot080317. ISSN 1559-6095

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Abstract or description

Proteomic approaches have been adopted to survey the degradome of caspases during apoptosis. These approaches provide a comprehensive list of substrates and give clues to which pathways are altered during apoptosis by activated caspases. However, substrates identified by large-scale proteomic screening need to be validated as bona fide caspase targets. This ensures that conclusions derived from the screen are based on real substrates and not on artifacts of the proteomic screen. The validation method described in this protocol uses radiolabeled versions of the putative substrates synthesized using in vitro transcription/translation methods. These are incubated with purified caspases to determine whether they are genuine caspase substrates.

Item Type:	Article
Faculty:	School of Life Sciences and Education > Biological Sciences
Depositing User:	Gavin MCSTAY
Date Deposited:	11 May 2018 13:18
Last Modified:	24 Feb 2023 13:50
URI:	https://eprints.staffs.ac.uk/id/eprint/4348

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