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Turnover of ATP synthase subunits in F1-depleted HeLa and yeast cells

Rak, Malgorzata, MCSTAY, Gavin, Fujikawa, Makoto, Yoshida, Masasuke, Manfredi, Giovanni and Tzagoloff, Alexander (2011) Turnover of ATP synthase subunits in F1-depleted HeLa and yeast cells. FEBS Letters, 585 (16). pp. 2582-2586. ISSN 00145793

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Abstract or description

Mitochondrial translation of the Saccharomyces cerevisiae Atp6p subunit of F(1)-F(0) ATP synthase is regulated by the F(1) ATPase. Here we show normal expression of Atp6p in HeLa cells depleted of the F(1) β subunit. Instead of being translationally down-regulated, HeLa cells lacking F(1) degrade Atp6p, thereby preventing proton leakage across the inner membrane. Mammalian mitochondria also differ in the way they minimize the harmful effect of unassembled F(1) α subunit. While yeast mutants lacking β subunit have stable aggregated F(1) α subunit in the mitochondrial matrix, the human α subunit is completely degraded in cells deficient in F(1) β subunit. These results are discussed in light of the different properties of the proteins and environments in which yeast and human mitochondria exist.

Item Type: Article
Faculty: School of Life Sciences and Education > Biological Sciences
Depositing User: Gavin MCSTAY
Date Deposited: 20 Mar 2019 10:33
Last Modified: 24 Feb 2023 13:55

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